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Nutrient Agar with Horse Serum (LMG Medium 38)

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]
Nutrient Agar with Horse Serum
(LMG Medium 38)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone......................................................................................... 5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Lab-Lemco beef extract................................................................1.0g
Horse serum...........................................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool medium to 45–50°C. Aseptically add 100.0mL of
sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of fastidious bacteria.
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