Nutrient Agar with Horse Serum

[所属分类:培养基配方] [发布时间:2021-6-23] [发布人:网站管理员2] [阅读次数:] [返回]

Nutrient Agar with Horse Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Horse serum...........................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source:  Nutrient agar is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se-
rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of Alysiella filiformis and
Simonsiella crassa.