Alkaline Bacillus Medium

[所属分类:培养基配方] [发布时间:2019-9-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Bacillus Medium
Composition per liter:
Agar............................................................................................15.0g
Peptone .......................................................................................10.0g
Glucose.......................................................................................10.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
Na 2 CO 3 solution ....................................................................100.0mL
pH 8.5–11.0 at 25°C
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.0g
Preparation of Na 2 CO 3 Solution: Add Na 2 CO 3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except Na 2 CO 3 solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Gently
heat while stirring and bring to boiling. Autoclave for 15 min at 10 psi
pressure–115°C. Cool to 45°–50°C. Aseptically add sterile Na 2 CO 3 so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of alkalophilic microorgan-
isms such as Bacillus alcalophilus, Bacillus circulans, and other Bacil-
lus species.