Alkaline Cellulose Agar

[所属分类:培养基配方] [发布时间:2019-9-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alkaline Cellulose Agar
Composition per liter:
Solution A..............................................................................900.0mL
Solution B..............................................................................100.0mL
Solution A:
Composition per 900.0mL:
Agar ............................................................................................15.0g
Cellulose powder MN 300..........................................................15.0g
NH 4 NO 3 ........................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
Peptone..........................................................................................1.0g
Yeast extract..................................................................................0.5g
CaCl 2 .............................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.4g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.0
with 1N HCl. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
Na 2 CO 3 .........................................................................................0.5g
Preparation of Solution B:  Add 0.5g of Na 2 CO 3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH
to 9.4 with 6% NaHCO 3 solution. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°–55°C.
Preparation of Medium: Aseptically combine 900.0mL of solu-
tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of cellulose-utilizing bacte-
ria.