Oat Flakes Agar with Yeast Extract (DSMZ Medium 248)

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oat Flakes Agar with Yeast Extract
(DSMZ Medium 248)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oat flakes....................................................................................20.0g
Agar............................................................................................12.0g
Yeast extract..................................................................................2.0g
Trace elements solution.............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Preparation of Medium: Add oat flakes to 500.0mL distilled/de-
ionized water. Gently heat and bring to boiling. Boil for 20 min. Filter
through cheese cloth. Add agar and yeast extract to the filtrate and
bring volume to 1.0L with distilled/deionized water. Mix thoroughly.
Add 1.0mL trace elements solution. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Streptomyces pur-
pureus=Kitasatoa purpurea and Thermoactinomyces putidus.