Oat Flakes Agar with Yeast Extract

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oat Flakes Agar
with Yeast Extract

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oat flakes....................................................................................20.0g
Agar............................................................................................12.0g
Yeast extract..................................................................................2.0g
Trace elements solution SL-6 ....................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add oat flakes to distilled/deionized wa-
ter and bring volume to 990.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 10 min. Filter through cheese-
cloth. Add remaining components to filtrate and bring volume to 1.0L
with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of actinomycetes.