Oatmeal Agar with Lupine Stems

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oatmeal Agar with Lupine Stems

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oatmeal.......................................................................................30.0g
Agar............................................................................................20.0g
Lupine stems........................................................................... variable
Preparation of Medium: Add oatmeal to 1.0L of distilled/deion-
ized water. Gently heat and bring to boiling. Continue boiling for 20
min. Strain through muslin. Bring volume of filtrate to 1.0L with dis-
tilled/deionized water. Add 20.0g of agar. Gently heat and bring to boil-
ing. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes. Cut green lupine
stems into 1.0cm pieces. Place 6 to 9 lupine stem pieces in tubes with
3.0mL of distilled/deionized water. Autoclave for 15 min at 15 psi
pressure–121°C. Melt agar tubes by gently heating. Cool to 50°C.
Aseptically add 1 sterile lupine stem to each tube. Allow to solidify in
a slanted position.
Use: For the cultivation and maintenance of Ascochyta fabae, Asco-
chyta pinodes, Ascochyta pisi, Ascochyta punctata, Ascochyta viciae-
pannonicae, Ascochyta viciae-villosae, and Colletotrichum orbiculare.