Oatmeal Agar with Lupine Stems (OA-LUP)

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oatmeal Agar with Lupine Stems
(OA-LUP)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
Oatmeal, rolled oats....................................................................10.0g
Glycerin .....................................................................................5.0mL
Lactic acid..................................................................................0.2mL
Lupine stems........................................................................... variable
Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use:  For the cultivation of Ceratocystis brunnea, Ceratocystis major,
and Ceratocystis radicicola.