OAY Agar

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

OAY Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Oatmeal.......................................................................................20.0g
Agar ............................................................................................18.0g
Yeast extract..................................................................................1.0g
Trace salts solution ....................................................................1.0mL
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring the volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add oatmeal to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Steam for 20 min. Filter through cheesecloth. Add agar. Add
sufficient distilled/deionized water to bring volume to 999.0mL. Gen-
tly heat and bring to boiling. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 1.0mL of sterile trace salts solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Actinomadura citrea, Actinomadura coerulea,
Actinomadura cremea, Actinomadura kijaniata, Actinomadura rubrobru-
nea, Actinomadura vinacea, Actinomadura viridis, Actinoplanes species,
Glycomyces harbinensis, Microbispora rosea, Micromonospora species,
Microstreptospora cinerea, Microtetraspora africana, Microtetraspora
fastidiosa, Microtetraspora ferruginea, Microtetraspora roseola, Micro-
tetraspora rosevolacea, Microtetraspora rubra, Microtetraspora salmo-
nea, Saccharothrix coeruleofusca, Saccharothrix longispora, Spiril-
lospora rubra, Streptomyces species, Streptosporangium pseudovulgare,
Streptosporangium sibiricum, and Thermoactinomyces vulgaris.