Oceanithermus Medium (DSMZ Medium 1149)

[所属分类:培养基配方] [发布时间:2021-6-25] [发布人:网站管理员2] [阅读次数:] [返回]

Oceanithermus Medium
(DSMZ Medium 1149)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Yeast extract..................................................................................2.0g
K 2 HPO 4 .......................................................................................0.78g
KH 2 PO 4 .......................................................................................0.75g
NH 4 Cl .........................................................................................0.53g
MgCl 2 ·6H 2 O ...............................................................................0.36g
CaCl 2 ·2H 2 O ................................................................................0.33g
NaNO 3 ..........................................................................................0.2g
Na 2 SO 4 ........................................................................................0.14g
Bicarbonate solution..............................................................100.0mL
Trace elements solution...........................................................10.0mL
Vitamin solution ......................................................................10.0mL
Bicarbonate Solution :
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 6.5. Filter sterilize.
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.....................................................................12.8g
FeCl 3 ·6H 2 O.................................................................................1.35g
NaCl..............................................................................................1.0g
NiCl 2 ·6H 2 O.................................................................................0.12g
MnCl 2 ·4H 2 O .................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
ZnCl 2 .............................................................................................0.1g
Na 2 SeO 3 ·5H 2 O..........................................................................0.026g
CuCl 2 ·2H 2 O..............................................................................0.025g
CoCl 2 ·6H 2 O..............................................................................0.024g
Na 2 MoO 4 ·2H 2 O........................................................................0.024g
H 3 BO 3 .........................................................................................0.01g
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate .....................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except bicarbonate so-
lution and vitamin solution, to distilled/deionized water and bring vol-
ume to 890.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add bicarbonate and vi-
tamin solutions. Mix thoroughly. Aseptically distribute into tubes or
flasks.
Use: For the cultivation of Oceanithermus spp.