Ogawa Egg Medium

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Ogawa Egg Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Chicken eggs, whole..............................................................200.0mL
Sodium glutamate-KH 2 PO 4 solution.....................................100.0mL
Glycerol .....................................................................................6.0mL
Malachite Green (2.0% solution)...............................................6.0mL
pH 6.8 ± 0.2 at 25°C
Sodium Glutamate-KH 2 PO 4 Solution:
Composition per 100.0mL:
Sodium glutamate.........................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Preparation of Sodium Glutamate-KH 2 PO 4 Solution: Add
components to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Soak eggs with 1:100 dilution of saturat-
ed mercuric chloride solution for 1 min. Aseptically break eggs into a
sterile graduated cylinder. Homogenize eggs. Add remaining compo-
nents. Mix thoroughly. Aseptically distribute into sterile tubes in
10.0mL volumes. Inspissate at 90°C (moist heat) for 60 min.
Use: For the selective isolation and cultivation of Nocardia and Rho-
dococci species.