Ogawa TB Medium

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Ogawa TB Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 300.0mL:

KH 2 PO 4 .........................................................................................1.0g
Homogenized whole egg .......................................................200.0mL
Glycerol .....................................................................................6.0mL
Malachite Green (2% solution)..................................................6.0mL
pH 6.5 ± 0.2 at 25°C
Homogenized Whole Egg:
Composition per liter:
Whole eggs ................................................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Preparation of Medium: Add components, except homogenized
whole egg, to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 200.0mL of sterile homogenized whole
egg. Mix thoroughly. Aseptically distribute into sterile screw-capped
tubes in 7.0mL volumes. Inspissate at 85°–90°C (moist heat) for 60
min.
Use:  For the isolation and cultivation of Mycobacterium species,
except for Mycobacterium leprae.