Oil Agar Medium

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Oil Agar Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar, purified..............................................................................20.0g
NaCl............................................................................................10.0g
Oil powder..................................................................................10.0g
NH 4 NO 3 ........................................................................................1.0g
MgSO 4 ..........................................................................................0.5g
Amphotericin B solution..........................................................10.0mL
K 2 HPO 4 solution........................................................................7.0mL
KH 2 PO 4 solution........................................................................3.0mL
FeCl 3 ..........................................................................................0.1mL
Oil Powder:
Composition  per 10.0g:
Hydrocarbon...............................................................................10.0g
Silica gel .....................................................................................10.0g
Diethyl ether ............................................................................30.0mL
Preparation of Oil Powder: Add 10.0g of hydrocarbon to 30.0mL
of diethyl ether. Mix thoroughly. Add 10.0g of silica gel. Allow ether
to evaporate.
Amphotericin B Solution:
Composition per 10.0mL:
Amphotericin B ..........................................................................0.01g
Preparation of Amphotericin B Solution: Add amphotericin B
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
K 2 HPO 4 Solution:
Composition per 100.0mL:
K 2 HPO 4 .......................................................................................10.0g
Preparation of K 2 HPO 4 Solution: Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
KH 2 PO 4 Solution:
Composition per 100.0mL:
KH 2 PO 4 .......................................................................................10.0g
Preparation of KH 2 PO 4 Solution: Add KH 2 PO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components—except amphotericin
B solution, K 2 HPO 4 solution, and KH 2 PO 4 solution—to distilled/de-
ionized water and bring volume to 980.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile amphot-
ericin B solution, 7.0mL of sterile K 2 HPO 4 solution, and 3.0mL of ster-
ile KH 2 PO 4 solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and enumeration of hydrocarbon-utilizing
bacteria by direct plating of estuarine water and sediment samples.