OM-2 Medium (DSMZ Medium 782)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

OM-2 Medium
(DSMZ Medium 782)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NH 4 -oxalate ................................................................................15.0g
NaHCO 3 ......................................................................................10.0g
NaH 2 PO 4 ·2H 2 O...........................................................................10.0g
NaS 2 O 3 ·5H 2 O................................................................................1.0g
NaCl..............................................................................................0.7g
KCl............................................................................................. 0.57g
MgCl 2 ·6H 2 O..................................................................................0.1g
CaCl 2 ·2H 2 O.................................................................................0.01g
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add NH 4 -oxalate, NaH 2 PO 4 ·2H 2 O, and
NaHCO 3 to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly after adding each until dissolved. Add remaining compo-
nents. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20
min at 15 psi pressure–121°C.
Use: For the cultivation of Ammoniphilus oxalivorans and Ammoniphi-
lus oxalaticus.