ONE Broth-Listeria (Oxoid Novel Enrichment (ONE) Broth-Listeria)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

ONE Broth-Listeria
(Oxoid Novel Enrichment (ONE) Broth-Listeria)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1005.0mL:

Peptone........................................................................................28.0g
Salt mix.......................................................................................10.0g
Carbohydrate mix ........................................................................6.0g
ONE Broth-Listeria selective supplement (proprietary) ...........5.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components, except ONE Broth-
Listeria selective supplement, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 5.0mL ONE Broth-Listeria selective supple-
ment. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective enrichment of Listeria spp. from food and envi-
ronmental samples. A selective enrichment broth for Listeria species
from food samples in 24 hr.