Önöz Salmonella Agar

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Önöz Salmonella Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sucrose........................................................................................13.0g
Lactose........................................................................................11.5g
Trisodium citrate–5,5–hydrate......................................................9.3g
Meat peptone ................................................................................6.8g
Beef extract...................................................................................6.0g
L– Phenylalanine............................................................................5.0g
Na 2 S 2 O 3 ·5H 2 O............................................................................4.25g
Bile salt mixture........................................................................3.825g
Yeast extract..................................................................................3.0g
Na 2 HPO 4 ·2H 2 O.............................................................................1.0g
Ferric citrate..................................................................................0.5g
Metachrome Yellow....................................................................0.47g
MgSO 4 ·7H 2 O................................................................................0.4g
Aniline Blue................................................................................0.25g
Neutral Red...............................................................................0.022g
Brilliant Green......................................................................0.00166g
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Salmonella from feces.