Oral Fusobacterium Medium

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Oral Fusobacterium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Proteose peptone.........................................................................10.0g
Na 2 HPO 4 .......................................................................................5.0g
Glucose.........................................................................................5.0g
Beef extract...................................................................................3.0g
Soluble starch................................................................................2.0g
NaNO 3 ..........................................................................................1.0g
Yeast extract..................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
Ethyl Violet solution................................................................10.0mL
Bacitracin solution...................................................................10.0mL
pH 7.6 ± 0.2 at 25°C
Ethyl Violet Solution:
Composition per 10.0mL:
Ethyl Violet.................................................................................0.04g
Preparation of Ethyl Violet Solution: Add Ethyl Violet to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Bacitracin Solution:
Composition per 10.0mL:
Bacitracin...................................................................................1.0mg
Preparation of Bacitracin Solution: Add bacitracin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except Ethyl Violet so-
lution and bacitracin solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile Ethyl Violet solution and bacitracin solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation and cultivation of oral Fusobacterium
species, especially Fusobacterium nucleatum.