Oral Treponema Medium

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Oral Treponema Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1250.0mL:

Veal heart, fresh ground.............................................................1.0Kg
Thiopeptone................................................................................20.0g
NaCl............................................................................................10.0g
Ionagar No. 2 ................................................................................2.0g
Glutathione (1% solution)......................................................100.0mL
Rabbit serum or ascitic fluid..................................................100.0mL
Eggs, whole fresh...............................................................................2
pH 6.8–7.0 at 25°C
Preparation of Medium: Add agar to 50.0mL of distilled/deion-
ized water. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. In a separate flask, add fine-
ly ground veal heart to 1.0L of distilled/deionized water. Add remain-
ing components, except glutathione, rabbit serum, and agar. Gently
heat and bring to 70°C. Adjust pH to 7.4. Gently heat and bring to
100°C. Continue heating at 100°C for 2 hr. Skim off fat. Filter through
glass wool. Adjust pH to 7.6. Gently heat and bring to 100°C. Maintain
at 100°C for 30 min. Store at 4°C for 18 hr. Sterilize in an Arnold ster-
ilizer for 30 min at 100°C on 2 consecutive days. Cool to 45°–50°C.
Aseptically add rabbit serum, glutathione solution, and sterile cooled
agar solution. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the isolation and cultivation of Treponema denticola and
Treponema oralis.