Photobacterium Broth

[所属分类:培养基配方] [发布时间:2021-7-7] [发布人:网站管理员2] [阅读次数:] [返回]

Photobacterium Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
Sodium glycerol phosphate.........................................................23.5g
Pancreatic digest of casein............................................................5.0g
KH 2 PO 4 .........................................................................................3.0g
Yeast extract..................................................................................2.5g
CaCO 3 ...........................................................................................1.0g
NH 4 Cl ...........................................................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.3g
FeCl 3 ...........................................................................................0.01g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks to form a shallow layer of medium. Autoclave for 15 min at
15 psi pressure–121°C.
Use:  For the cultivation and demonstration of luminescence by photo-
bacteria. For the cultivation and maintenance of Alteromonas hanedai,
Photobacterium phosphoreum, Shewanella hanedai, Vibrio fischeri,
Vibrio harveyi, and other Vibrio species.