Phthalic Acid Medium

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Phthalic Acid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................400.0mL
Solution 2...............................................................................400.0mL
Potassium hydrogen phthalate
solution............................................................................200.0mL
pH 6.8 ± 0.2 at 25°C
Solution 1:
Composition per 400.0mL:
KH 2 PO 4 .........................................................................................9.1g
(NH 4 ) 2 SO 4 ....................................................................................1.2g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 6.8
with KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Solution 2:
Composition per 400.0mL:
MgSO 4 ·7H 2 O................................................................................0.4g
FeSO 4 ·7H 2 O................................................................................0.01g
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 6.8
with KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Potassium Hydrogen Phthalate Solution:
Composition per 200.0mL:
Potassium hydrogen phthalate......................................................1.0g
Preparation of Potassium Hydrogen Phthalate Solution:
Add potassium hydrogen phthalate to distilled/deionized water and
bring volume to 200.0mL. Mix thoroughly. Adjust pH to 6.8 with
KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine the three sterile so-
lutions. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation and maintenance of Pseudomonas cepacia.