PHYG Medium

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

PHYG Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 110.1mL:

Beef heart, solids from infusion..................................................10.0g
Polypeptone™...............................................................................2.0g
Gelatin...........................................................................................1.0g
Glucose .........................................................................................1.0g
Yeast extract..................................................................................1.0g
NaHCO 3 ........................................................................................0.5g
Tryptose ........................................................................................0.2g
Agar ............................................................................................0.16g
NaCl..............................................................................................0.1g
L -Cysteine·HCl·H 2 O ...................................................................0.09g
(NH 4 ) 2 SO 4 ...................................................................................0.05g
Resazurin .................................................................................0.16mg
Salts solution............................................................................50.0mL
Rabbit serum, inactivated ........................................................10.0mL
Thiamine pyrophosphate solution..............................................0.1mL
pH 7.2–7.5 at 25°C
Salts Solution:
Composition per 400.0mL:
K 2 HPO 4 .........................................................................................0.9g
NaCl..............................................................................................0.8g
KH 2 PO 4 .........................................................................................0.4g
MnCl 2 ·4H 2 O................................................................................0.16g
MgSO 4 ........................................................................................0.08g
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 400.0mL. Mix thoroughly.
Thiamine Pyrophosphate Solution:
Composition per 10.0mL:
Thiamine pyrophosphate.............................................................0.05g
Preparation of Thiamine Pyrophosphate Solution: Add thia-
mine pyrophosphate to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except rabbit serum
and thiamine pyrophosphate solution, to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL of sterile rabbit serum and 0.1mL of
sterile thiamine pyrophosphate solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of treponemes.