Pilobolus Agar

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pilobolus Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Sodium acetate............................................................................10.0g
Yeast extract..................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 ..................................................................................0.66g
MgSO 4 ·7H 2 O................................................................................0.5g
Thiamine..................................................................................10.0mg
Hemin ......................................................................................10.0mg
NaOH (0.1N solution)..............................................................37.5mL
pH 8.0 ± 0.2 at 25°C
Preparation of Medium: Add hemin to NaOH solution and mix
thoroughly to dissolve. Add remaining components and bring volume
to 1.0L with distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and maintenance of Pilobolus species.