Pilobolus Medium (DSMZ Medium 192)

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pilobolus Medium
(DSMZ Medium 192)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Na-acetate...................................................................................10.0g
Yeast extract..................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 ..................................................................................0.66g
MgSO 4 ·7H 2 O................................................................................0.5g
Thiamine-HCl·2H 2 O................................................................10.0mg
Hemin solution........................................................................37.5 mL
pH 8.0 ± 0.2 at 25°C
Hemin Solution:
Composition per 10.0mL:
Hemin.......................................................................................10.0mg
Preparation of Hemin Solution: Add hemin to 37.5 mL 0.1N
NaOH. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.0.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Pilobolus sphaerosporus.