Pirellula marina Medium M14

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pirellula marina Medium M14

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................1.0g
Yeast extract..................................................................................1.0g
Artificial seawater..................................................................680.0mL
Tris·HCl buffer,
(0.1M solution, pH 7.5).....................................................50.0mL
Hutner's basal salts...................................................................20.0mL
Vitamin solution.......................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl..........................................................................................23.47g
MgCl 2 ·6H 2 O..............................................................................10.64g
Na 2 SO 4 ........................................................................................3.92g
CaCl 2 .............................................................................................1.1g
KCl.........................................................................................664.0mg
NaHCO 3 .................................................................................192.0mg
KBr...........................................................................................96.0mg
H 3 BO 3 ......................................................................................26.0mg
SrCl 2 .........................................................................................24.0mg
NaF.............................................................................................3.0mg
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Nicotinamide..............................................................................9.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Cyanocobalamin ........................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solution,
to distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Pirellula marina.