Pisu Medium

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pisu Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1512.0mL:

Agar base...............................................................................960.0mL
Horse serum, sterile...............................................................360.0mL
L -Cystine solution..................................................................180.0mL
Lead acetate solution...............................................................12.0mL
pH 6.8 ± 0.2 at 25°C
Agar Base:
Composition per liter:
Proteose peptone No. 3...............................................................20.0g
Agar..............................................................................................7.0g
NaCl..............................................................................................5.0g
Meat extract..................................................................................4.0g
Preparation for Agar Base: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Dissolve in
steam for 15 min at 0 psi pressure–100°C. Cool to 45°–50°C. Adjust
pH to 7.5. Filter sterilize. Distribute into 200.0mL Erlenmeyer flasks
in 80.0mL volumes. Autoclave for 60 min at 0 psi pressure–100°C.
Cool to 60°C.
L -Cystine Solution:
Composition per 200.0mL:
L- Cystine.......................................................................................2.0g
Preparation of  L -Cystine Solution: Add  L -cystine to distilled/de-
ionized water and bring volume to 200.0mL. Mix thoroughly. Filter
sterilize.
Lead Acetate Solution:
Composition per 100.0mL:
Lead acetate................................................................................10.0g
Preparation of Lead Acetate Solution: Add lead acetate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: To each flask containing 80.0mL of
cooled, sterile agar base, aseptically add 30.0mL of sterile horse serum,
15.0mL of sterile  L -cystine solution, and 1.0mL of sterile lead acetate
solution. Mix thoroughly. Aseptically distribute into small sterile tubes
in 2.0–3.0mL volumes.
Use: For the cultivation and differentiation of bacteria based on their
ability to produce cystinase. Cystinase-producing bacteria turn the
medium black.