PKU Test Agar (Phenylketonuria Test Agar)

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

PKU Test Agar
(Phenylketonuria Test Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
K 2 HPO 4 .......................................................................................15.0g
Glucose .......................................................................................10.0g
KH 2 PO 4 .........................................................................................5.0g
(NH 4 )Cl.........................................................................................2.5g
(NH 4 )NO 3 .....................................................................................0.5g
Asparagine....................................................................................0.5g
DL- Alanine.....................................................................................0.5g
L- Glutamic acid.............................................................................0.5g
Na 2 SO 4 ..........................................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.05g
FeCl 3 ..........................................................................................5.0mg
MnCl 2 ·4H 2 O...............................................................................5.0mg
β-2-Thienylalanine.....................................................................3.3mg
CaCl 2 ·2H 2 O................................................................................2.5mg
Bacillus subtilis spore suspension............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 5 min. Do not autoclave. Cool to 50°C.
Add 10.0mL of a suspension of Bacillus subtilis ATCC 6633 spores.
Mix thoroughly. Pour into sterile Petri dishes or other containers.
Use: For the determination of phenylalanine concentrations in serum
or urine. Used in the Guthrie-modified bacterial-inhibition assay pro-
cedure for screening newborn infants for phenylketonuria (PKU).