PKU Test Agar (Phenylketonuria Test Agar)

[所属分类:培养基配方] [发布时间:2021-7-8] [发布人:网站管理员2] [阅读次数:] [返回]

PKU Test Agar
(Phenylketonuria Test Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .......................................................................................15.0g
Agar............................................................................................13.5g
Glucose.........................................................................................5.0g
KH 2 PO 4 .........................................................................................5.0g
(NH 4 )Cl.........................................................................................2.5g
L- Asparagine.................................................................................0.5g
L- Glutamic acid.............................................................................0.5g
Na 2 SO 4 ..........................................................................................0.5g
(NH 4 )NO 3 .....................................................................................0.5g
L- Alanine.....................................................................................0.25g
MgSO 4 ·7H 2 O..............................................................................0.05g
FeCl 3 .........................................................................................0.005g
MnSO 4· ......................................................................................0.005g
CaCl 2 ·2H 2 O ...............................................................................2.5mg
β-2-Thienylalanine solution.......................................................1.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
β-2-Th i enylalanine Solution:
Composition per 100.0mL:
β-2-Thienylalanine .....................................................................0.33g
Preparation of β-2-Th i enylalanine Solution: Add β-2-thienyla-
lanine to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except β-2-thienylala-
nine solution, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Continue boiling for
5 min. Do not autoclave. Cool to 50°C. Add 10.0mL of a suspension of
Bacillus subtilis ATCC 6633 spores and 1.0mL of sterile β-2-thienyla-
lanine solution. Mix thoroughly. Pour into sterile Petri dishes or other
containers.
Use: For the determination of phenylalanine concentrations in serum
or urine. Used in the Guthrie-modified bacterial-inhibition assay pro-
cedure for screening newborn infants for phenylketonuria (PKU).