Plates with Fluoranthene (DSMZ Medium 462a)

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Plates with Fluoranthene
(DSMZ Medium 462a)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1052.0mL:

Agar ............................................................................................15.0g
Na 2 HPO 4 .....................................................................................2.44g
KH 2 PO 4 .......................................................................................1.52g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Fluoranthene solution ..............................................................50.0mL
Trace elements solution SL-4 ..................................................10.0mL
Vitamin solution.........................................................................2.5mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxamine.............................................................................5.0mg
Vitamin B 12 ................................................................................2.0mg
Nicotinic acid.............................................................................2.0mg
p-Aminobenzoate.......................................................................1.0mg
Thiamine-HCl·2H 2 O.................................................................1.0mg
Ca-pantothenate.........................................................................0.5mg
Biotin.........................................................................................0.2mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Fluoranthene Solution:
Composition per 100.0mL:
Fluoranthene...............................................................................10.0g
Preparation of Fluoranthene Solution: Add fluoranthene to
100.0mL acetone. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except vitamin solution
and fluoranthene solution, to 1.0L distilled/deionized water. Adjust pH
to 7.1. Gently heat and bring to boil. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°C. Aseptically add 2.5mL vitamin solution. Mix
thoroughly. Pour into sterile Petri dishes (20.0mL per Petri dish). Allow
plates to dry for 48 hr. Aseptically spread 1.0mL of fluoranthene solu-
tion onto the surface of the dried plates. Allow the acetone to evaporate
under sterile conditions.
Use: For the cultivation of Mycobacterium gilvum, Sphingomonas sp.
(Sphingomonas paucimobilis), unclassified bacterium (Gordona sp.),
Mycobacterium sp., Sphingomonas sp., and other biphenyl-utilizing
bacteria.