PLET Agar

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

PLET Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................500.0g
Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Ethylenediamine tetracetic acid (EDTA)......................................0.3g
Thallous acetate..........................................................................0.04g
Antibiotic inhibitor..................................................................10.0mL
Antibiotic Inhibitor:
Composition per 10.0mL:
Lysozyme.............................................................................300,000U
Polymyxin..............................................................................30,000U
Preparation of Antibiotic Inhibitor:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhib-
itor, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically add sterile antibiotic
inhibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the selective isolation and cultivation of Bacillus anthracis.