Pluton Medium

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Pluton Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Glucose .......................................................................................10.0g
Malt extract...................................................................................5.0g
Neopeptone...................................................................................5.0g
Peptone..........................................................................................2.5g
Yeast extract..................................................................................2.5g
Starch ............................................................................................2.0g
Trypticase™..................................................................................2.0g
Potassium phosphate buffer (1M solution, pH 7.2) .................50.0mL
L -Cysteine·HCl solution...........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl............................................................................0.25g
Preparation of  L -Cysteine·HCl Solution: Add  L -cysteine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Add components, except  L -cysteine·HCl
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Distribute 10.0mL volumes into tubes. Autoclave for
15 min at 15 psi pressure–121°C. Aseptically add 0.1mL of sterile  L -
cysteine·HCl solution to each tube. Mix thoroughly.
Use:  For the cultivation of Melissococcus pluton.