PM Indicator Agar

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

PM Indicator Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................5.25g
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Pancreatic digest of casein............................................................1.7g
Sorbitan monooleate complex ......................................................1.0g
NaCl..............................................................................................0.5g
Papaic digest of soybean meal......................................................0.3g
K 2 HPO 4 .......................................................................................0.25g
Bromcresol Purple ......................................................................0.06g
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Inoculate medium with a suspension of
Bacillus stearothermophilus spores. Pour into 100mm flat-bottomed
Petri dishes in 6.0mL volumes. Use medium immediately.
Use: For the rapid detection of trace amounts of penicillin in milk using
the AOAC Bacillus stearothermophilus Qualitative Disc Method II.