Polymyxin Pyruvate Egg Yolk Mannitol Bromthymol Blue Agar (PEMBA)

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Polymyxin Pyruvate Egg Yolk Mannitol
Bromthymol Blue Agar
(PEMBA)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 110.0mL:

Agar..............................................................................................1.8g
D -Mannitol....................................................................................1.0g
Sodium pyruvate...........................................................................1.0g
Na 2 HPO 4 .....................................................................................0.25g
NaCl..............................................................................................0.2g
Peptone .........................................................................................0.1g
KH 2 PO 4 .....................................................................................0.025g
Bromthymol Blue.......................................................................0.01g
MgSO 4 ·7H 2 O..............................................................................0.01g
Antibiotic inhibitor....................................................................5.0mL
Egg yolk emulsion (20% solution)............................................5.0mL
pH 7.4 ± 0.2 at 25°C
Antibiotic Inhibitor:
Composition per 5.0mL:
Polymyxin B.......................................................................... 10,000U
Preparation of Antibiotic Inhibitor:  Add components to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Fil-
ter sterilize.
Egg Yolk Emulsion, 20% Solution:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 20% Solution: Soak eggs
with 1:100 dilution of saturated mercuric chloride solution for 1 min.
Crack eggs and separate yolks from whites. Mix egg yolks with 1
chicken egg. Measure 20.0mL of egg yolk emulsion and add to 80.0mL
of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–
50°C.
Preparation of Medium: Add components, except antibiotic inhib-
itor and egg yolk emulsion, 20%, to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile antibiotic inhibitor and egg yolk emulsion, 20%.
Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of Bacillus cereus.