Polymyxin Staphylococcus Medium

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Polymyxin Staphylococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Lecithin.........................................................................................0.7g
Polymyxin.................................................................................0.075g
Tween™ 80..............................................................................10.2mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the selective isolation and cultivation of pathogenic, coagu-
lase-positive Staphylococcus aureus. Proteus species will grow on this
medium but appear as translucent colonies.