Polypectate Gel Medium

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Polypectate Gel Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium polypectate.....................................................................70.0g
K 2 HPO 4 .........................................................................................5.0g
Peptone..........................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 ·2H 2 O...................................................................................0.6g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium:  Add components, except sodium polypec-
tate, to 500.0mL of boiling water. Put into a blender and mix on low speed
to dissolve. Add the sodium polypectate slowly with the blender on low
speed to minimize air bubbles. Adjust pH to 7.0. Bring volume to 1.0L
with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Pour into sterile Petri dishes.
Use: For the cultivation of microoganisms and detection of pectate-
degrading enzymes.