Pontecorvo’s Aspergillus Medium with 0.05% Yeast Extract

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Pontecorvo’s Aspergillus Medium
with 0.05% Yeast Extract

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................30.0g
NaNO 3 ..........................................................................................6.0g
KH 2 PO 4 .......................................................................................1.52g
KCl..............................................................................................0.52g
MgSO 4 ·7H 2 O..............................................................................0.52g
Yeast extract..................................................................................0.5g
ZnSO 4 .......................................................................................0.001g
FeCl 3 ........................................................................................0.85mg
Biotin........................................................................................40.0μg
Trace metals solution.................................................................1.0mL
pH 6.2 ± 0.2 at 25°C
Trace Metals Solution:
Composition per liter:
CuSO 4 ·5H 2 O...............................................................................0.25g
Na 2 B 4 O 7 ·10H 2 O............................................................................0.1g
MnCl 2 ·4H 2 O ............................................................................50.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................50.0mg
Preparation of Trace Metals Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.2 with KOH. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Aspergillus nidulans.