Porcine Heart Agar

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Porcine Heart Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Porcine heart, infusion from.....................................................375.0g
Agar............................................................................................15.0g
Papaic digest of soybean meal......................................................6.5g
Glucose.........................................................................................5.0g
NaCl..............................................................................................5.0g
Proteose peptone No. 3.................................................................5.0g
Yeast extract..................................................................................3.5g
Sheep blood, defibrinated........................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep
blood. Mix thoroughly. Pour into sterile Petri dishes.
Use: For determination of the sensitivity of microorganisms using the
disc plate technique.