Potassium Cyanide HiVeg Broth Base with KCN

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Potassium Cyanide HiVeg Broth Base with KCN

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................5.64g
NaCl..............................................................................................5.0g
Plant peptone No. 3.......................................................................3.0g
KH 2 PO 4 .....................................................................................0.225g
KCN solution...........................................................................15.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without potassium cyanide solution, is avail-
able as a premixed powder from HiMedia.
KCN Solution:
Composition per 100.0mL:
KCN..............................................................................................0.5g
Preparation of KCN Solution: Add KCN to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Caution: Cyanide is toxic.
Preparation of Medium: Add components, except KCN solution,
to distilled/deionized water and bring volume to 985.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add 15.0mL of KCN solu-
tion. Mix thoroughly. Distribute into sterile screw-capped tubes or
flasks in 1.0–1.5mL volumes. Close caps tightly.
Use: For the cultivation and differentiation of urease-negative, Gram-
negative enteric bacteria. Salmonella species and Shigella species are
nonmotile in this medium. Proteus species are motile in this medium.