Potassium Tellurite Agar

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Potassium Tellurite Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................500.0g
Agar ............................................................................................15.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Blood, defibrinated ..................................................................50.0mL
K 2 TeO 3 solution.......................................................................20.0mL
pH 6.0 ± 0.2 at 25°C
K 2 TeO 3 Solution:
Composition per 20.0mL:
K 2 TeO 3 ..........................................................................................0.5g
Preparation of K 2 TeO 3 Solution: Add K 2 TeO 3 to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril-
ize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except K 2 TeO 3 solu-
tion, to distilled/deionized water and bring volume to 930.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
K 2 TeO 3 solution and 50.0mL of blood. Rabbit or sheep blood may be
used. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes. Allow tubes to cool in a slanted position.
Use: For the cultivation and differentiation of Enterococcus faecalis.
Enterococcus faecalis appears as black colonies.