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Potato Agar
[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]
Potato Agar
Agar ............................................................................................20.0g
Preparation of Medium: Dice potatoes and place in 1.0L of tap
water. Gently heat and bring to boiling. Boil potatoes until thoroughly
cooked. Filter through cheesecloth. Bring volume of filtrate to 1.0L
with tap water. Add 20.0g of agar. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Cercospora medicaginis,
Metarhizium anisopliae, Metarhizium flavoviride, Phoma chrysan-
themi, Phoma eupyrena, Phoma exigua, Phoma foveata, Phoma
lingam, Phoma macrostoma, Phoma pinodella, Phoma putaminum,
Polyscytalum pustulans, Sclerotium cepivorum, Ulocladium atrum,
Ulocladium botrytis, Ulocladium chartarum, Ulacladium corsortiale,
and Ulocladium curcurbitae.
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Potatoes.....................................................................................200.0gAgar ............................................................................................20.0g
Preparation of Medium: Dice potatoes and place in 1.0L of tap
water. Gently heat and bring to boiling. Boil potatoes until thoroughly
cooked. Filter through cheesecloth. Bring volume of filtrate to 1.0L
with tap water. Add 20.0g of agar. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Cercospora medicaginis,
Metarhizium anisopliae, Metarhizium flavoviride, Phoma chrysan-
themi, Phoma eupyrena, Phoma exigua, Phoma foveata, Phoma
lingam, Phoma macrostoma, Phoma pinodella, Phoma putaminum,
Polyscytalum pustulans, Sclerotium cepivorum, Ulocladium atrum,
Ulocladium botrytis, Ulocladium chartarum, Ulacladium corsortiale,
and Ulocladium curcurbitae.