Potato Agar with Cereal Culms

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Agar with Cereal Culms

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes.....................................................................................200.0g
Agar............................................................................................20.0g
Cereal culms ........................................................................... variable
Preparation of Medium: Peel and dice potatoes. Add potatoes to
1.0L of tap water. Gently heat and bring to boiling. Boil potatoes until
thoroughly cooked. Filter through cheesecloth. Bring volume of filtrate
to 1.0L with tap water. Add 20.0g of agar. Gently heat and bring to boil-
ing. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Cut cereal culms into 1.0cm pieces. Place 6 to 9 cereal culm
pieces in tubes with 3.0mL of distilled/deionized water. Autoclave for
15 min at 15 psi pressure–121°C. Melt agar tubes by gently heating.
Cool to 50°C. Aseptically add 2 to 3 sterile cereal culm pieces to each
tube. Allow to solidify in a slanted position.
Use: For the cultivation of fungi.