Potato Carrot Medium

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Carrot Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter of tap water:

Potatoes, sliced with skin..........................................................300.0g
Carrots, peeled and sliced...........................................................25.0g
Agar............................................................................................15.0g
Preparation of Medium:  Slice potatoes with the skin on. Peel car-
rots and slice. Add potatoes and carrots to approximately 700.0mL of
tap water. Gently heat and bring to boiling. Continue boiling for 20
min. Filter through cheesecloth. Bring volume of filtrate to 1.0L with
distilled/deionized water. Add agar. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Actinoplanes awajinensis,
Actinoplanes nirasakinensis, Amorphosporangium auranticolor, Strep-
tomyces flaveus, and Thermoactinomyces vulgaris.