Potato Dextrose Adenine Agar (ATCC Medium 2204)

[所属分类:培养基配方] [发布时间:2021-7-9] [发布人:网站管理员2] [阅读次数:70] [返回]

Potato Dextrose Adenine Agar
(ATCC Medium 2204)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Potatoes, infusion from..........................................................500.0mL
Adenine solution......................................................................50.0mL
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion:  Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Adenine Solution:
Composition  per 50.0mL:
Adenine.........................................................................................0.1g
Preparation of Adenine Solution: Add adenine to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except adenine solu-
tion, to distilled/deionized water and bring volume to 950.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of
sterile adenine solution. Mix thoroughly. Pour into sterile Petri dishes
or distriubte to sterile tubes.
Use: For the cultivation of yeasts and molds.