Potato Dextrose Agar

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Potato, infusion from ....................................................................4.0g
Tartaric acid solution................................................................14.0mL
pH 5.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Tartaric Acid Solution:
Composition per 50.0mL:
Tartaric acid ..................................................................................5.0g
Preparation of Tartaric Acid Solution: Add tartaric acid to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 986.0mL. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 14.0mL of sterile tar-
taric acid solution. Mix thoroughly. If medium is to be used for the enumer-
ation of yeasts and molds in butter, adjust pH to 3.5. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and enumeration of yeasts and molds. For the
enumeration of yeasts and molds in butter by the plate count method.