Potato Dextrose Agar

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Glucose .......................................................................................20.0g
Potato infusion.......................................................................200.0mL
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition per 10.0mL:
Potatoes, unpeeled and sliced ...................................................200.0g
Preparation of Potato Infusion: Add potato slices to 1.0L of dis-
tilled/deionized water. Gently heat and bring to boiling. Continue boil-
ing for 30 min. Filter through cheesecloth. Reserve filtrate.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and enumeration of yeasts and filamentous
fungi (molds) from foods.