Potato Dextrose Agar (BAM M127)

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar
(BAM M127)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Glucose.......................................................................................20.0g
Potato infusion..............................................................................1.0L
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition per liter:
Potatoes, unpeeled and sliced...................................................200.0g
Preparation of Potato Infusion: Add unpeeled potato slices to
1.0L of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate. Bring volume to 1.0L with distilled/deionized water.
Preparation of Medium: Add agar and glucose to 1.0L potato in-
fusion. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and enumeration of yeasts and filamentous
fungi (molds) from foods.