Potato Dextrose Agar with Antibiotics

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar with Antibiotics

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Potato, infusion from ....................................................................4.0g
Antibiotic solution ...................................................................20.0mL
Antibiotic Solution:
Composition per 100.0mL:
Chlortetracycline·HCl...................................................................0.5g
Chloramphenicol...........................................................................0.5g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of
sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri dish-
es.
Use: For the cultivation of fungi from foods.