Potato Dextrose Agar with Gentamicin (ATCC Medium 2286)

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Agar with Gentamicin
(ATCC Medium 2286)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Potatoes, infusion from..........................................................500.0mL
Gentamicin solution.................................................................10.0mL
pH 5.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Potato Infusion:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potato Infusion:  Peel and dice potatoes. Add
500.0mL of distilled/deionized water. Gently heat and bring to boiling.
Continue boiling for 30 min. Filter through cheesecloth. Reserve fil-
trate.
Gentamicin Solution:
Composition per 10.0mL:
Gentamycin............................................................................100.0mg
Preparation of Gentamicin Solution: Add gentamycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except gentamicin, to
distilled/deionized water and bring volume to 990.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile gen-
tamicin solution. Mix thoroughly. Pour into sterile Petri dishes or
sterile tubes.
Use: For the cultivation of yeasts and molds from dairy products and
other foods.