Potato Glucose Agar

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Glucose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potato, infusion from ................................................................500.0g
Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Preparation of Medium: Peel and slice potatoes thinly. Add
800.0mL of distilled/deionized water immediately to potatoes to pre-
vent oxidation. Gently heat and bring to 60°C. Maintain at 60°C for 60
min. Filter through cheesecloth. Adjust volume of filtrate to 1.0L with
distilled/deionized water. Add agar. Gently heat and bring to boiling.
Add glucose. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 20 min at 10 psi pressure–115°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Nocardia asteroides,
Pseudomonas caryophylli, Pseudomonas syringae, Rhodococcus species,
Streptomyces nobilis, Streptomyces prasinosporus, and Streptomyces spe-
cies.