Potato Infusion Agar

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Infusion Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes, infusion from.............................................................200.0g
Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Proteose peptone.........................................................................10.0g
Beef extract...................................................................................5.0g
NaCl..............................................................................................5.0g
Glycerol ...................................................................................20.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Potatoes, Infusion From:
Composition per 500.0mL:
Potatoes.....................................................................................300.0g
Preparation of Potatoes, Infusion From:  Peel and dice potatoes.
Add 500.0mL of distilled/deionized water. Gently heat and bring to
boiling. Continue boiling for 30 min. Filter through cheesecloth.
Preparation of Medium: Add glycerol to 500.0mL of distilled/de-
ionized water. Add remaining components. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the isolation of Brucella abortus.