Potato Infusion with Inorganic Salts

[所属分类:培养基配方] [发布时间:2021-7-12] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Infusion with Inorganic Salts

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potato........................................................................................200.0g
Agar............................................................................................15.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
CaCl 2 · 2H 2 O...................................................................................0.1g
ZnSO 4 · 7H 2 O...............................................................................0.03g
MnSO 4 ·5H 2 O..............................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
FeSO 4 ·7H 2 O................................................................................0.01g
Preparation of Medium:  Peel and dice potatoes. Add 500.0mL of
distilled/deionized water. Gently heat and bring to boiling. Continue
boiling for 30 min. Filter through cheesecloth. Bring volume of filtrate
to 1.0L. Add agar. Mix thoroughly. Gently heat and bring to boiling.
Add remaining components. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Bacillus macquariensis.